منابع مشابه
Killing K Channels with TEA (dekalificationychannel blockydecyltriethylammoniumytetraethylammonium)
Tetraethylammonium (TEA1) is widely used for reversible blockade of K channels in many preparations. We noticed that intracellular perfusion of voltage-clamped squid giant axons with a solution containing K1 and TEA1 irreversibly decreased the potassium current when there was no K1 outside. Five minutes of perfusion with 20 mM TEA1, followed by removal of TEA1, reduced potassium current to <5% ...
متن کاملExtracellular Blockade of K+ Channels by Tea
TEA is a classical blocker of K(+) channels. From mutagenesis studies, it has been shown that external blockade by TEA is strongly dependent upon the presence of aromatic residue at Shaker position 449 which is located near the extracellular entrance to the pore (Heginbotham, L., and R. MacKinnon. 1992. Neuron. 8:483-491). The data suggest that TEA interacts simultaneously with the aromatic res...
متن کاملTEA-insensitive K-channels in the crab giant axon.
TTX and TEA-insensitive permeabilities were studied in the crab giant axon under voltage-clamp. Membrane currents in the presence of internal TEA (40 mmol/l) and external TTX (300 nmol/l) may be analyzed as the sum of two components: a linear component, identified as the so-called leakage current, and a non-linear component, identified as a TEA-insensitive potassium channel. Ion permeability ra...
متن کاملExtracellular Blockade of K Channels by TEA: Results from Molecular Dynamics Simulations of the KcsA Channel
TEA is a classical blocker of K channels. From mutagenesis studies, it has been shown that external blockade by TEA is strongly dependent upon the presence of aromatic residue at Shaker position 449 which is located near the extracellular entrance to the pore (Heginbotham, L., and R. MacKinnon. 1992. Neuron. 8:483– 491). The data suggest that TEA interacts simultaneously with the aromatic resid...
متن کاملBlock of mouse Slo1 and Slo3 K+ channels by CTX, IbTX, TEA, 4-AP and quinidine
pH-regulated Slo3 channels, perhaps exclusively expressed in mammalian sperm, may play a role in alkalization-mediated K(+) fluxes associated with sperm capacitation. The Slo3 channel shares extensive homology with Ca(2+)- and voltage-regulated BK-type Slo1 K(+) channels. Here, using heterologous expression in oocytes, we define distinctive differences in pharmacological properties of Slo3 and ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.24.13335